Collagen is a major component of connective tissues, and its regeneration is crucial for repairing ligaments, tendons, muscles, and even blood vessels
Amino acid substitutions at the dimer interface of human glucose-6-phosphate dehydrogenase that increase thermostability and reduce the stabilising effect of NADP
Nat Commun 9:4934
For humans, self-production of B12 through the intestinal bacteria is theoretically possible, but rarely achieved for health reasons, which is why humans today depend on a dietary intake of B12 in almost all cases
The bacterial expression plasmid, which expresses glutathione S-transferase (GST)-labeled CRIP1, its mutants GST-CRIP1 R16K and GST-CRIP1 R26/68K, as well as the truncated forms GST-CRIP1 (1-57aa) and GST-CRIP1 (58-77aa), was prepared by inserting the target DNA fragments into the pGEX-6P-1 vector