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and 5 mM DTT
The purification method used in these protocols does not require use of phenol
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C
surfactants
Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
L-Histidine, monohydrochloride, monohydrate size:100g and 5 mM DTT
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