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Summary: Vitamin B12 toxicity from supplementation is extremely rare, but persistently elevated serum levels may indicate underlying liver disease, kidney dysfunction, or haematological disorders requiring medical investigation

Those with digestive issues, like Crohns disease or celiac disease, that impair nutrient absorption

The frequency of B12 shots can vary based on individual needs and health goals

Any animal-model protocol should include defined humane endpoints, veterinary oversight, and IACUC or equivalent ethics-committee approval before initiation

All current evidence comes from preclinical animal and cellular studies, meaning its efficacy and safety in people remain unproven