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Histochemical and immunohistochemical staining Tissue cryosections were fixed for 15 min, incubated with SA--gal staining at 37 C overnight in the dark according to the manufacturers instructions (Solarbio, Beijing, China)

Thermogravimetric analysis (TGA) was carried out on a Perkin-Elmer TGA instrument, from room temperature to 900 C at a heating rate of 10 C min 1 under N 2 atmosphere

10.1016/S0891-5849(01)00480-4 Free Radic

Transformed cells were cultured in LB medium at 37 C until reaching an OD 600 of 0.50.6, then induced with 0.5 mM IPTG at 16 C for 1618 h

49 FauconA.FuE.StengelB.MazharF.EvansM.CarreroJ