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We selected pAcF as the handles for constructing conjugates instead of other chemistry such as azide mediated click chemistry as the cyclopropane-tetrazine ligation employed in the pAcF system demonstrates superior bioorthogonality compared to azide-alkyne cycloadditions, effectively minimizing off-target reactions in complex cellular environments

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Glutathione binds to harmful toxins, heavy metals, and free radicals, neutralizing them before they can cause damage

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