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De novo glutathione synthesis is catalyzed by the heterodimeric glutamate cysteine ligase (GCL), which is composed of catalytic (GCLC) and modifier (GCLM) subunits (Lu 2013)

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Additionally, the pocket normally occupied by the conserved phenylalanine residue of the TIN2 F-X-L-X-P motif was fully obstructed by a crystal contact with a TRF1 TRFH copy in an adjacent asymmetric unit, further limiting the possibility of obtaining XChem hits outside of the leucine pocket

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