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However, the UK Department of Health (1991) has derived its recommendations for folate intake in pregnancy at 300 g/d (69) using evidence available at that time from several historic intervention studies with different folic acid doses (Reference Hansen and Rybo70,Reference Chanarin, Rothman and Ward71) but there are some concerns that the requirements might be underestimated as folate intake through the diet in these studies has not been accurately measured