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Determining the total glutathione and amount of GSSG are performed in parallel reactions

[U- 13 C]glutamine isotope tracing experiments showed that GGCT overexpression reduced Gln contribution to the TCA cycle and diverted it preferentially to the GSH synthesis pathway for ROS regulation

Quantification statistical analysis Values are expressed as mean standard error of the mean (s.e.m.) from at least three independent experiments or biological replicates, unless otherwise indicated in the figure legend

Mitochondria as multifaceted regulators of ferroptosis