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Our bodies are amazing machines, but sometimes they throw us curveballs

Dietary polyphenols and the prevention of diseases

Cells (1 10 6 ) were lifted and washed with cold PBS and resuspended in 100 l binding buffer according to the manufacturers protocol (BD, FITC Annexin V Apoptosis Detection kit I)

Protein band intensity was quantified using the ImageJ program (rsbweb.nih.gov/ij/download.html) and normalized with -actin (for total homogenate) or lamin-A (for nuclear extracts)

82 It has also been discovered that variations in the amino acid sequences, 83 transcription, and efficiency of this gene secretion affect the amounts of vacuolating activity