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reported that the NO has a biphasic effect on testosterone secretion, NO inhibits testosterone secretion at higher levels and stimulant at low concentrations

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The mice were injected intravenously with PBS, Ru(II)-OH, NP2, NP4 (2 mg/kg Ru) on days 1, 4, 7, 10, respectively

In PTSD mice models, trauma exposure increased tyrosine hydroxylase expression (the rate-limiting enzyme in catecholamine biosynthesis), elevating stress hormones epinephrine and norepinephrine in serum and colon, which upregulated claudin-2 expression, confirming gut barrier dysfunction (Yadav et al., 2023)