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After each step, a buffer wash was performed for 5 min using EnVisionTM FLEX wash Buffer (20 ) (Agilent, K800721-2, Santa Clara, CA, USA) [32]

PSA, a protein produced by prostate cells, is frequently present at elevated levels in the blood of men who have prostate cancer

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However, absence of identified source and standardized extraction and purification protocols have remarkably influenced the application of polysaccharides

Glutathione as a Substrate: Why GSH Supply Matters Too GSTM1 and GSTT1 enzyme activity depends on two things: the presence of the enzymes themselves (determined by deletion status) and an adequate supply of reduced glutathione (GSH) as the conjugation substrate