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HEK293T cells ( Homo sapiens , female, embryonic kidney cells, ATCC, CRL-1573) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 g/ml streptomycin (1% Biochrome, 0257 F) and 2 mM sodium pyruvate (1% Biochrome, L 0473)
However, experts warn that GHK-Cu Powder should never be mixed with strong vitamin C (L-ascorbic acid), retinol, or EDTA to prevent oxidation and loss of activity
After thorough washout, cells were treated with RSL3 (250 nM) for 2 h
Treatments: Anti-Aging Hyperpigmentation Glutathione is an active principle that plays an important role in nutrition and the regulation of certain cellular events such as gene expression
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