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Mutations in GNE lead to the neuromuscular disorder, GNE myopathy, characterized by muscle weakness and atrophy, with protein aggregation seen in muscle biopsy samples 3

10.1093/burnst/tkac038 31 HomaeigoharS.MonavariM.KoenenB.BoccacciniA

Glutathione: Overview of its protective roles, measurement, and biosynthesis Moderne oversiktsartikkel om glutathions biologiske funksjoner, syntese og kliniske relevans

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The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication