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Flow cytometry analysis Annexin-V-FITC test for the externalization of phosphatidylserine to the outer side of the lipid bilayer and the EMA test, which shows the transformation of band 3 transmembrane protein, were performed as following: after the incubation of RBCs with tBuOOH for 45 h, the aliquots RBC 5 10 6 cells/mL were stained with Annexin-V (0.1 g/mL, 15 min, 25 C

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