2023), approximately 20 mg of lipids were extracted from freeze-dried yeast cells and analyzed using a UPLC 30A system (Shimadzu, Japan) equipped with a Kinetex C18 column (100 mm 2.1 mm, 2.6 m, Phenomenex, America) and coupled to a Triple TOF 6600 system (AB Sciex, Canada)
Upon reaching 80% confluency, cells were washed with cold PBS and mechanically scraped into 1 mL of ice-cold Chelex-100-treated PBS containing 0.05% v/v benzonase (Sigma
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The same trend was observed in trophozoite-stage parasites