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To further improve the transient assay, two types of infiltration media [10 mM MgCl 2 , 5 mM MES-KOH (pH 5.6), and 1.6 mM AS] and [0.5 MS medium, 5 mM MES-KOH (pH 5.6) and 1.6 mM AS], modified according to reported [29], were compared, which gave very similar results in our experiments (Fig

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Yoder KK, Territo PR, Hutchins GD, Hannestad J, Morris ED, Gallezot JD et al (2015)