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The ability of this mutant enzyme to incorporate certain 2´-modified-dNTPs (including but not limited to dNTPs having a 2´-fluorine or 2´-amino group) enables efficient in vitro synthesis of transcripts composed of mixed rNMP/2´-dNMP or rNMP/2´- modified-dNMP from any DNA template that is downstream of the T7 RNA polymerase promoter
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PCR amplification was performed under standard conditions using three different lots of EconoTaq DNA Polymerase and buffer
Phage T1-Resistant TransforMax™ EPI300™-T1RE
and Keller
Betaine hydrochloride size:250g The ability of this mutant
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