RNA 5' Polyphosphatase, 200 U size:EACH and the yellow dye migrates

and the yellow dye migrates at 75 bp

blunt ends or recessed 3' ends

The enzyme is supplied with a 10X Reaction Buffer

Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets)

Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity

RNA 5' Polyphosphatase, 200 U size:EACH and the yellow dye migratesDephosphorylates 5 triphosphorylated RNA, such as primary RNA transcripts, but does not desphosphorylate 5 capped mRNA Specific: Removes the and phosphates from 5 triphosphorylated and the phosphates from diphosphorylated RNA, but will not dephosphorylate monophosphorylated or 5 capped RNA Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Flexible: Use for various applications such as 5 RNA ligation tagging using T4 RNA Ligase,