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4 represents SDS-PAGE of lung microsomal proteins of normal guinea pigs and guinea pigs exposed to smoke from cigarettes with and without charcoal filter, wherein, the activated charcoal filter was constructed by placing stipulated amounts of different grain sizes or mixture of grain sizes of activated charcoal in a thin plastic tube, the inside diameter of which was same as the outside diameter of the tobacco portion of the cigarette or the conventional cellulose acetate filter