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The concentrations and mean peak size of the libraries were determined by Qubit 4.0 fluorometer and TapeStation system, respectively, and libraries were placed onto Illumina Novaseq 6000 (Illumina Way, San Diego, CA) for cluster generation and sequencing

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The extreme heat leads to hyperkeratosis or thickening of the tissue similar to a skin callus and is not regarded as being premalignant

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