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Similarly, HAEC cells were cultured in 6-well plates at a density of 2.0 10 5 cells/well, starved overnight, and treated with 50 mg/ml ENDS liquid with nicotine or ENDS liquid without nicotine for 24 hours or 1 mM H 2 O 2 for 12 hours (positive control) and then subjected to Annexin V/PI apoptosis analysis by flow cytometry