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Samples were amplified on a CFX-96 Bio-Rad real time PCR machine using SsoAdvanced universal probes supermix (1725284, Bio-Rad) and TaqMan primers for PARP-1 and GAPDH as housekeeping gene (Hs00242302_m1 resp

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That is, for every 100 cigarettes consumed there, another 67 were smuggled out