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RNAScope FISH (tissue and ALI section histological labeling) Adjacent cross-sections of human trachea or PBS rinsed ALI cultures were fixed in 10% neutral buffered formalin for 24 8 h at room temperature, washed with 1 PBS and paraffin-embedded immediately

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[DOI] [PMC free article] [PubMed] [Google Scholar] [266].Zhong J, Agha G, Baccarelli AA, The Role of DNA Methylation in Cardiovascular Risk and Disease: Methodological Aspects, Study Design, and Data Analysis for Epidemiological Studies, Circulation research, 118 (2016) 119131

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