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We did not observe this effect with the same concentration of nicotine alone in the short-term experiments, but we could observe the dose-dependent inhibitory effect of nicotine on cellular metabolism already at 50 ng/mL and 100 ng/mL in the long-term exposure (21 days)

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Further investigations showed that in vitro pretreatments with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a major carcinogen component of tobacco, impaired the expansion of cytotoxic T lymphocytes (CTLs) following their transfer into mice but elevated the frequency of precursor memory CLTs, resulting in a final moderate decline in memory CLTs [91]