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For the dissociation phase, the sensors were put in sybody-free buffer again

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Causes / Risk Factors High energy food intake and lack of physical activity Dysbiosis in infanthood (may be a result of diet or antibiotic use) Chronic otitis media (may increase preferences for sweeter and saltier junk foods) Childhood stress Parental BMI overweight parents doubles the risk for overweight children (genes and obesegenic environment) Maternal smoking during pregnancy Gestational Diabetes Nutritional deficiency in pregnancy, especially methylating factors, folic acid and vitamin B12 Low birth weight Not breast feeding

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To confirm the abundance of abundance of major taxa, ileal and colonic mucous samples were collected and frozen and stored at 80 C freezer immediately