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Classification 1: Classify by the principle of smoke emission Heat-not-burn e-cigarettes: The main principle is low-temperature heating, which releases smoke by heating a new type of tobacco, also referred to as HNB

Quantitative Real Time-PCR analysis Due to the difficulty of growing large numbers of mouse AEC cells, quantitative real-time RT-PCR, which only requires small amounts of RNA, was chosen to determine mouse iNOS mRNA levels and -actin (internal standard)

Psychological processing in chronic pain: A neural systems approach

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