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To calculate relative mRNA expressions, data were normalized to -actin expression, and the relative expression levels were quantified using the comparative CT method

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Finally, before moving on to the stage of enamel, each object must be cleaned with brushes and pickling acids with special care because, especially working the silver, not all the metal oxide glazes turn out to be compatible with the oxide of silver, for which it is necessary to eliminate it completely or at least with extreme care to avoid direct contact with the metal of certain colors, interposing a layer of transparent enamel