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In contrast, if metabolically competent human HepaRG cells [142] or primary human hepatocytes [46] were treated with 20 mM APAP for 24 h, there was 8090% GSH depletion, a ten-fold higher protein adducts formation, 7090% reduction of the mitochondrial membrane potential and 610-fold increase in LDH or ALT release

As ROS levels rise, they damage the delicate lipid membranes and mitochondrial DNA, impairing the electron transport chain

Nutrition Research, 99 , 4050

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In the enrichment sub-cultures, the communitys nutritional requirements were met by either compounds dissolved in the medium itself (Table 1) biomass or byproducts of other community members [e.g., exuded organic carbon (Christie-Oleza et al., 2017