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ARG2 duplex RT-qPCR was performed on HL-60 cells treated with or without nicotine in presence or absence of natural compound library ( n = 124) using Taqman Gene Expression Cells-to-CT kit as per manufacturers recommendation on Bio-Rad CFX connect

The new data from the Health Behavior in School-aged Children (HBSC) study also highlights a narrowing gender gap in substance use, emphasizing the need for targeted prevention strategies