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Cell culture Caco-2 epithelial colon cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and expanded in Dulbeccos modified Eagles medium (DMEM) supplemented with 20% FBS (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) to promote optimal growth and viability

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The data reached an all-time high of 20,280.000 Ton in Mar 2016 and a record low of 6,199.000 Ton in Nov 2015

Three months into treatment, 21% of participants had stopped drinking altogether