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RNA quality was assessed by electrophoresis in a 1% agarose gel to check for genomic DNA contamination and degradation, and further quantified using a Kaiao K5500 spectrophotometer (Kaiao, Beijing, China) and an Agilent 2100 Bioanalyzer (Agilent Technologies, California, USA)

The study also mentions this, noting that independent stores complied at a much lower rate in comparison to chain stores

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By letting the tobacco sit, you reduce its moisture content, allowing for a more even and consistent burn