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They have: Primary site: The designed cleavage site Secondary sites: Weaker recognition sequences elsewhere in your protein Non-specific activity: Especially at high concentrations or long incubations Thrombin is the worst offender: Research on thrombin-induced degradation found that "while thrombin and factor Xa are quite specific for cleavage at the inserted cleavage site, proteolysis can frequently occur at other site(s) in the protein of interest." The problem is compounded by commercial thrombin preparations containing secondary protease activity from contaminating proteases
After this crisis was managed, the main event was held at Forte di Belvedere, located on the highest hill of the Boboli Gardens (from which the pizza gets its name)
In addition, as the MI is exogenous in classic models, we attempted to consider the MI as an exogenous sector in this research
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