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This review summarizes results from studies of humans, preclinical animal models, and cultured endothelial cells that document toxicities of cigarette smoke exposure on pulmonary endothelial cell functions, including barrier dysfunction, endothelial activation and inflammation, apoptosis, and vasoactive mediator production

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A total of 2 mL of saliva was collected from each participant and stored in labeled sterile cryovials with the addition of Halt Protease and Phosphatase Inhibitor cocktail (1% v/v, ThermoScientific, Waltham, MA) and stored at 80 C for downstream untargeted high-resolution HPLCMS metabolic analysis as previously described 33,34,35,36