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A 1:3 dilution was performed on red blood cell samples by using a 5% PCA/BA solution

However, this is more to do with how little the peptide has been researched

Cunningham FX, Gantt E (2007) A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study

Environ Toxicol Pharmacol 43:182192 Marchiani A, Rozzo C, Fadda A, Delogu G, Ruzza P (2014) Curcumin and curcumin-like molecules: from spice to drugs

Microbial fermentation yields adenosylcobalamin, which is then converted to cyanocobalamin by the addition of potassium cyanide or thiocyanate in the presence of sodium nitrite and heat