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Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature

Most importantly, designing reliable clinical trials featuring high-quality, large sample sizes, randomized, double-blind controls, and other rigorous methodologies is crucial for evaluating and developing effective interventions

We propose an integrative framework centered on the iron dyshomeostasis-ferroptosis axis, aiming to transcend the traditional linear cascade hypothesis

However, the specifics of choice of supplement, dosing and duration of treatment should be individualized through discussion with Dr