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do not rinse After 4 to 8 weeks of good tolerance, consider increasing to 2 to 3 percent or twice daily Commit to a minimum of 3 months before assessing response, and 6 months for a full evaluation Combining topical GHK-Cu with weekly or biweekly microneedling at 0.5 mm to 1.0 mm can substantially improve follicular penetration

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References Vitamin B12 Product Label Cyanocobalamin injection [package insert]

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After that, its efficacy can diminish, and the risk of contamination climbs with every passing day and every additional puncture