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A systematic review and meta-analysis of the sex ratio for Barretts esophagus, erosive reflux disease, and nonerosive reflux disease

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Cell pellets resuspended in BEGM + 0.5 PSA were filtered using a 70 m cell strainer, collected by centrifugation (5 min, 225 g , 4 C) and cryopreserved in freeze media (F-media, 30% FBS, 10% DMSO)