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ZNF, zinc finger transcription factors

Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions

Following washes, membranes were incubated for 1 h at room temperature with either IRDye 680RD Donkey anti-Mouse IgG secondary antibody at 1:2000 dilution (Licor, 926-68072) or IRDye 800CW Donkey anti-Rabbit IgG secondary antibody at 1:3000 dilution (Licor, 926-32213)