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Such an effect was observed also in the case of nicotine: in the study by Zanetti et al., apoptosis induction in primary alveolar cells was observed at 100 nM nicotine, whereas 10 M nicotine was needed to exert a similar effect in the mouse MLE-12 lung epithelial cell line, and human BEAS-2B bronchial epithelial cells appeared to be even more resistant to nicotine (Zanetti et al