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We were interested in testing how varenicline altered the long-lasting effects of nicotine on nAChRs and determining whether this process involved nAChRs inside of cells. In the brain, there is an upregulation (an increase in number and sensitivity) of nAChRs as they adapt to chronic nicotine

In IL-17A group, the cells were induced by 50 ng/ml IL-17A (Biolegend, CA, USA)

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