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Cell death was assessed using propidium iodide (PI) red fluorescent probe to quantify the proportion of PI-positive cells, as previously reported 53

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PMID 2189310

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The study further showed that restoring mitochondrial biogenesis through PGC-1 overexpression enhances T cell metabolic fitness and anti-tumor function, highlighting the critical role of metabolic regulation in determining T cell efficacy in the TME ( Moreover, hypoxic tumor cells reprogram their antioxidant systems to cope with elevated ROS levels