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The slides were then rinsed with PBS buffer and mounted with a 1:1 (v/v) PBS/glycerol solution before being stored at 4 C overnight

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Smokers are seven to ten times more likely to suffer from oral cancer than people who have never smoked, with long-term, regular users of smokeless tobacco being more than 11 times more at risk than a non-user

[10] The term oxidative stress refers to the total of the intracellular and extracellular conditions that lead to chemical or metabolic production of reactive oxygen species (ROS)

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