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The fourth ring (upper treatment boundary line) was located 5 cm above the second ring and indicated the ending boundary line for the treatment

Coming in to FSU, I knew that research was something I wanted to experience but I was not sure in what way

Maintenance Reality: What Youll Actually Need to Do Heres the honest maintenance schedule based on my three years of testing: Weekly/Bi-Weekly: Vacuum or wipe the pre-filter (takes 2 minutes) Check air quality indicator if present Monthly: Wash washable pre-filters (WINIX, some Alen models) Inspect for unusual odors indicating filter saturation Every 6-12 Months: Replace main filter on most units Reset filter indicator light Every 1-5 Years: Replace HEPA/carbon filters on long-life units (Austin Air, IQAir) Pro tip: Set a phone reminder for monthly pre-filter cleaning

Check the model to see how many square feet it can handle

During the exposure, basolateral perfusion was maintained with culture media (DMEM high glucose:LHC basal media, 1:1 ratio) at 37 C and 5% CO 2