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Regardless, patients with TDP-43 mutations have TDP-43 pathology, and the mouse model that have A315T mutation has TDP-43 accumulations mostly in the nucleus, have extensive ubiquitinated protein accumulations in the cytoplasm, and based on the antibody used, pTDP-43 are also detected in the cytoplasm 19,46

A lifted skinfold technique (pinching or bunching the skin) can be used to lift the subcutaneous layer away from the underlying muscle (Down and Kirkland, 2012) (Fig 3)

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Current Pharmaceutical Design, 24(18), 19471956