Collect the banded cells in the upper layer, dilute with Solution B and centrifuge at 700 g for 10 min to pellet the motoneuron fraction
For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
O.WarzokR.HachenbergT.KauffmannH
As reported in the cases of PVY NTN and TuMV, the GSH form and total glutathione levels could be upregulated in susceptible plants until the point of symptom occurrence and then highly depleted (Otulak-Kozie et al., 2022b, 2023)
A retrospective study investigated changes in cognitive function and cerebral metabolism-related indicators in DKD patients