Blind cage coding and automated video analysis (EthoVision, TopScan) are recommended Too low n : prior power analysis, n 8 minimum for rodent behavioural tests No positive control : without memantine, donepezil or scopolamine depending on the paradigm, impossible to judge if the model is functional No dose-response : a single tested dose is insufficient to conclude
If you want a closer look at a mineral-focused routine, Why We Made It: Magnesium Complex is a useful next read
20 Detection Methods There are numerous methods for detecting glutathione, with commonly used ones including the iodometric method, the DTNB (Ellman's reagent) method, the sodium nitroprusside method, the fluorometric method, the alloxan method, and high-performance liquid chromatography (HPLC)
As glutathione decreases, the lining of the blood vessels becomes less pliable and more resistant to widening when necessary for increasing oxygen transfer to tissues
National Center for Biotechnology Information, "D-glutamate"