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At high nutrient and ATP levels, AMPK activity is inhibited, and mTORC1 phosphorylates ULK1 and ATG13L of the autophagy-activating protein complex and thus suppresses autophagy [267, 268]

The cell pellets were cultured in chondrogenic medium (low-glucose Dulbeccos modified Eagle medium containing 0.1 mmol/L ascorbic acid 2-phosphate, 100 nmol dexamethasone, 40 g/mL proline, 100 U/mL penicillin, 100 g/mL streptomycin, and ITS Premix

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Add a small amount of water, massage and rinse thoroughly with lukewarm water