Aliquots of proteins from L02 cells were pre-purified with Protein A/G beads (#B23202, Bimake, TX, USA) in the presence of non-specific IgG (Santa Cruz) and then incubated with His-tag antibody and 50 L beads overnight at 4 C with continuous mixing
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Glutathione oxidized Based on 9 publication(s) in Google Scholar Glutathione oxidized (L-Glutathione oxidized) is produced by the oxidation of glutathione
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Furthermore, alanine mutations of the established glutathione-scaffold site residue D90 or of crucial residue K26 in monothiol PfGrx C32S/C88S slowed down the reaction and resulted in linear secondary diagrams for the first rate constant (Fig