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Acta Biomater (2022) 140:2342

As the first step, the primers for HrGSTm1 (forward primer: 5-CGGCTACTGGGACATTCG-3

In brief, 0.2 L urine samples were analyzed by SDS-PAGE (10% gels) under reducing or non-reducing conditions and then transferred to polyvinylidene fluoride [PVDF (Immobilon -P Transfer Membrane, Merk Millipore Ltd., Burlington, MA, United States)] membranes by wet blotting (Mini Trans-Blot Electrophoretic Transfer Cell, BioRad Laboratories, Inc., Hercules, CA, United States)

This analysis also demonstrated the merits of a minimal systemic toxic response and a paucity of therapeutic side effects [107, 108]

J Biol Chem 272 :74457454