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Iron-catalyzed lipid peroxidation, along with iron-catalyzed protein nitration, contributes to the induction of the mitochondrial permeability transition and cell necrosis

Do not use cloudy, discolored, or particle-containing solution unless the official instructions say otherwise

Ethics declarations Competing interests The corresponding author, Holly Stankewicz, has recently started doing some consulting for Galephar Pharmaceuticals for a hangover medication

Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

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